Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC One-step TUNEL FITC Apoptosis Detection Kit 20rxns
One-step TUNEL FITC Apoptosis Detection Kit can be used to detect nuclear DNA fragmentation in tissues or cells undergoing apoptosis During apoptosis intracellular DNA endonucleases become activated cleaving genomic DNA between nucleosomes into 180-200 bp fragments or multiples thereof The resulting 3 -OH DNA ends are labeled with tagged dUTP through the catalytic action of Terminal Deoxynucleotidyl Transferase (TdT) allowing detection via fluorescence microscopy or flow cytometry with excitation/emission wavelengths at Ex/Em 492 nm/517 nm This kit has broad application and can be used to detect apoptosis in frozen or paraffin-embedded tissue sections as well as in cultured adherent or suspension cells
Cambridge Isotope Laboratories 1.5 mM HIS6-GB1+10 mM PBS+0.02% NaN3+0.1 mM DSS 90%(V/V) H2O/D2O (U-13C, 99%; U-15N, 99%), 1 5MM TUBE
1.5 mM HIS6-GB1+10 mM PBS+0.02% NaN3+0.1 mM DSS 90%(V/V) H2O/D2O (U-13C, 99%; U-15N, 99%), 1 5MM TUBE
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Kd Medical Inc PBS 10X, PH 7.4, 500ML
10X PBS is concentrated is and ready to be diluted to a 1X working solution. Used as a wash buffer in several research applications. One 500ml bottle.
Teknova 1X PBS, Dulbecco's Solution (with Calcium and Magnesium). 1000mL, Sterile 2 pack.
137mM Sodium chloride, 0.5mM Magnesium chloride, 8.1mM Disodium phosphate, 0.9mM Calcium chloride, 1.47mM Monopotassium phosphate, 2.7mM Potassium chloride
Bioworld PBS-Casein, 0.5%, 200 mL
Supplied in PBS as 0.5% casein ready for use.pH range 7.4 (+/- 0.1)Sold as 2 x 100mL or 500mL.
Alkali Scientific CellPro™ PBS 1X, pH 7.4, withSodium Azide, FAcs Washing Grade, 20L Cubitainer
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Research Products International Corp Phosphate Buffered Saline, 10X Solution, 1 Liter
PBS 10X Solution is a concentrated phosphate-buffered saline widely used in cell biology, immunohistochemistry, and molecular biology. It contains phosphate ions that maintain physiological pH and ionic strength, creating a stable environment for cells, tissues, and biomolecules. The 10X concentration allows for easy dilution to 1X for various applications such as washing, resuspending, or preparing samples. The phosphate buffer system resists pH changes, ensuring experimental consistency. PBS 10X is a reliable, versatile reagent that supports reproducible results in biological and biochemical workflows.
Research Products International Corp PBS, 10X Powder Concentrate, 10 L
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
Alkali Scientific CellPro™ PBS 1X, pH 7.4, with Sodium Azide, FAcs Washing Grade, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Alkali Scientific CellPro™ PBS Buffer, 1X, pH 7.4, 1L
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
![Research Products International Corp PBS [Phosphate Buffered Saline] 100 ml Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P32080-100T.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline] 100 ml Tablets, 100 Tablets per Bottle
PBS, 100 mL Tablets are pre-weighed tablets used to prepare 100 mL of Phosphate-Buffered Saline, a widely used buffer in biological research. Each tablet contains sodium chloride, sodium phosphate dibasic, and potassium phosphate monobasic, which dissolve in water to form an isotonic, pH-balanced solution. PBS is used in cell culture, immunohistochemistry, protein and nucleic acid research, and diagnostic assays. These tablets ensure consistent buffer preparation, eliminate weighing steps, and save time in lab workflows. They support sample integrity and provide a stable environment for biological reactions.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Apexbio Technology LLC Live-Dead Cell Staining Kit I (Calcein AM/PI) 100T
Calcein AM/PI Live-Dead Cell Staining Kit is a reagent kit used to distinguish between viable mammalian cells with esterase activity and dead cells The kit contains two probes Calcein AM and Propidium Iodide (PI) Calcein AM is a probe that stains live cells it is a derivative of Calcein with an added AM moiety which makes it more hydrophobic and allows better cell permeability Calcein AM itself is non-fluorescent but once it enters live cells it is hydrolyzed by intracellular esterases into Calcein which can emit strong green fluorescence PI is a probe for dead cells which enters only cells with compromised membranes staining them red The fluorescence intensities of these two probes reflect cell viability and toxicity The kit is easy to use 30 minutes of staining incubation After staining results can be detected using fluorescence microscopy fluorescence microplate readers or flow cytometry This kit is unsuitable for bacteria or fungi